The s-Pro ßit, N-term plasmid encodes the ßit fragment of the split LuxSit™ Pro enzyme as an N-terminal reporter tag for a protein of interest. The ßit, N-term plasmid works with the LuxSit™ s-Pro Protein:Protein Interaction System and must be paired with a protein tagged with s-Pro αLux. The plasmid backbone is pcDNA3.1 with CMV as promoter. The gene of interest can be inserted in frame with the LuxSit enzyme using (BamHI, XhoI, SacI, EcoRI, EcoRV, BstXI, XbaI) restriction enzymes. The plasmid is shipped lyophilized in 25 ug aliquots.
Must be ordered with part NC3199859